John M. Flanagan All publications ( 35 ) Sudol M. Tran M. Nowak MG. Flanagan JM. Robertson GP. Katzman M. A nonradioactive plate-based assay for stimulators of nonspecific DNA nicking by HIV-1 integrase and other nucleases. 2009 Sep 11. Anal Biochem. . Purdy JG. Flanagan JM. Ropson IJ. Craven RC. Retroviral capsid assembly: a role for the CA dimer in initiation. 2009 Jun 5. J Mol Biol. 389(2):438-51. Bewley MC. Graziano V. Griffin K. Flanagan JM. Turned on for degradation: ATPase-independent degradation by ClpP. 2009 Feb. J Struct Biol. 165(2):118-25. Sundstrom JM. Tash BR. Murakami T. Flanagan JM. Bewley MC. Stanley BA. Gonsar KB. Antonetti DA. Identification and analysis of occludin phosphosites: a combined mass spectrometry and bioinformatics approach. 2009 Feb. J Proteome Res. 8(2):808-17. Zinnanti WJ. Lazovic J. Griffin K. Skvorak KJ. Paul HS. Homanics GE. Bewley MC. Cheng KC. Lanoue KF. Flanagan JM. Dual mechanism of brain injury and novel treatment strategy in maple syrup urine disease. 2009 Apr. Brain. 132(Pt 4):903-18. De Cotiis DA. Woll MP. Fox TE. Hill RB. Levenson R. Flanagan JM. Optimized expression and purification of myristoylated human neuronal calcium sensor 1 in E. coli. 2008 Oct. Protein Expr Purif. 61(2):103-12. Purdy JG. Flanagan JM. Ropson IJ. Rennoll-Bankert KE. Craven RC. Critical role of conserved hydrophobic residues within the major homology region in mature retroviral capsid assembly. 2008 Jun. J Virol. 82(12):5951-61. Chen KM. Spratt TE. Stanley BA. De Cotiis DA. Bewley MC. Flanagan JM. Desai D. Das A. Fiala ES. Amin S. El-Bayoumy K. Inhibition of nuclear factor-kappaB DNA binding by organoselenocyanates through covalent modification of the p50 subunit. 2007 Nov 1. Cancer Res. 67(21):10475-83. Carkaci-Salli N. Flanagan JM. Martz MK. Salli U. Walther DJ. Bader M. Vrana KE. Functional domains of human tryptophan hydroxylase 2 (hTPH2). 2006 Sep 22. J Biol Chem. 281(38):28105-12. National Institute of General Medical Sciences Bewley MC. Graziano V. Griffin K. Flanagan JM. The asymmetry in the mature amino-terminus of ClpP facilitates a local symmetry match in ClpAP and ClpXP complexes. 2006 Feb. J Struct Biol. 153(2):113-28. Howitt J. Bewley MC. Graziano V. Flanagan JM. Freimuth P. Structural basis for variation in adenovirus affinity for the cellular coxsackievirus and adenovirus receptor. 2003 Jul 11. J Biol Chem. 278(28):26208-15. National Institute of Allergy and Infectious Diseases Flanagan JM. Bewley MC. Protein quality control in bacterial cells: integrated networks of chaperones and ATP-dependent proteases. 2002. Genet Eng (N Y). 24:17-47. National Institute of General Medical Sciences Thiboutot D. Bayne E. Thorne J. Gilliland K. Flanagan J. Shao Q. Light J. Helm K. Immunolocalization of 5alpha-reductase isozymes in acne lesions and normal skin. 2000 Sep. Arch Dermatol. 136(9):1125-9. National Institute of Arthritis and Musculoskeletal and Skin Diseases Bayne EK. Flanagan J. Einstein M. Ayala J. Chang B. Azzolina B. Whiting DA. Mumford RA. Thiboutot D. Singer II. Harris G. Immunohistochemical localization of types 1 and 2 5alpha-reductase in human scalp. 1999 Sep. Br J Dermatol. 141(3):481-91. Bewley MC. Springer K. Zhang YB. Freimuth P. Flanagan JM. Structural analysis of the mechanism of adenovirus binding to its human cellular receptor, CAR. 1999 Nov 19. Science. 286(5444):1579-83. National Center for Research Resources Huang K. Flanagan JM. Prestegard JH. The influence of C-terminal extension on the structure of the "J-domain" in E. coli DnaJ. 1999 Jan. Protein Sci. 8(1):203-14. National Institute of General Medical Sciences Freimuth P. Springer K. Berard C. Hainfeld J. Bewley M. Flanagan J. Coxsackievirus and adenovirus receptor amino-terminal immunoglobulin V-related domain binds adenovirus type 2 and fiber knob from adenovirus type 12. 1999 Feb. J Virol. 73(2):1392-8. National Institute of Allergy and Infectious Diseases Huang K. Ghose R. Flanagan JM. Prestegard JH. Backbone dynamics of the N-terminal domain in E. coli DnaJ determined by 15N- and 13CO-relaxation measurements. 1999 Aug 10. Biochemistry. 38(32):10567-77. National Institute of General Medical Sciences Wang J. Hartling JA. Flanagan JM. Crystal structure determination of Escherichia coli ClpP starting from an EM-derived mask. 1998 Dec 15. J Struct Biol. 124(2-3):151-63. National Institute of General Medical Sciences Lupas A. Flanagan JM. Tamura T. Baumeister W. Self-compartmentalizing proteases. 1997 Oct. Trends Biochem Sci. 22(10):399-404. Wang J. Hartling JA. Flanagan JM. The structure of ClpP at 2.3 A resolution suggests a model for ATP-dependent proteolysis. 1997 Nov 14. Cell. 91(4):447-56. National Institute of General Medical Sciences Tolman JR. Flanagan JM. Kennedy MA. Prestegard JH. NMR evidence for slow collective motions in cyanometmyoglobin. 1997 Apr. Nat Struct Biol. 4(4):292-7. Szabo A. Korszun R. Hartl FU. Flanagan J. A zinc finger-like domain of the molecular chaperone DnaJ is involved in binding to denatured protein substrates. 1996 Jan 15. EMBO J. 15(2):408-17. National Institute of General Medical Sciences Tolman JR. Flanagan JM. Kennedy MA. Prestegard JH. Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution. 1995 Sep 26. Proc Natl Acad Sci U S A. 92(20):9279-83. National Institute of General Medical Sciences Shanklin J. DeWitt ND. Flanagan JM. The stroma of higher plant plastids contain ClpP and ClpC, functional homologs of Escherichia coli ClpP and ClpA: an archetypal two-component ATP-dependent protease. 1995 Oct. Plant Cell. 7(10):1713-22. Flanagan JM. Wall JS. Capel MS. Schneider DK. Shanklin J. Scanning transmission electron microscopy and small-angle scattering provide evidence that native Escherichia coli ClpP is a tetradecamer with an axial pore. 1995 Aug 29. Biochemistry. 34(34):10910-7. Hill RB. MacKenzie KR. Flanagan JM. Cronan JE. Prestegard JH. Overexpression, purification, and characterization of Escherichia coli acyl carrier protein and two mutant proteins. 1995 Aug. Protein Expr Purif. 6(4):394-400. National Institute of Allergy and Infectious Diseases National Institute of General Medical Sciences Hill RB. Flanagan JM. Prestegard JH. 1H and 15N magnetic resonance assignments, secondary structure, and tertiary fold of Escherichia coli DnaJ(1-78). 1995 Apr 25. Biochemistry. 34(16):5587-96. National Institute of General Medical Sciences Szabo A. Langer T. Schröder H. Flanagan J. Bukau B. Hartl FU. The ATP hydrolysis-dependent reaction cycle of the Escherichia coli Hsp70 system DnaK, DnaJ, and GrpE. 1994 Oct 25. Proc Natl Acad Sci U S A. 91(22):10345-9. National Institute of General Medical Sciences Flanagan JM. Kataoka M. Fujisawa T. Engelman DM. Mutations can cause large changes in the conformation of a denatured protein. 1993 Oct 5. Biochemistry. 32(39):10359-70. National Institute of General Medical Sciences Engelman DM. Adair BD. Brünger A. Flanagan JM. Hunt JF. Lemmon MA. Treutlein H. Zhang J. Dimerization of glycophorin A transmembrane helices: mutagenesis and modeling. 1993. Soc Gen Physiol Ser. 48:11-21. Flanagan JM. Kataoka M. Shortle D. Engelman DM. Truncated staphylococcal nuclease is compact but disordered. 1992 Jan 15. Proc Natl Acad Sci U S A. 89(2):748-52. Lemmon MA. Flanagan JM. Treutlein HR. Zhang J. Engelman DM. Sequence specificity in the dimerization of transmembrane alpha-helices. 1992 Dec 29. Biochemistry. 31(51):12719-25. National Institute of General Medical Sciences Langer T. Lu C. Echols H. Flanagan J. Hayer MK. Hartl FU. Successive action of DnaK, DnaJ and GroEL along the pathway of chaperone-mediated protein folding. 1992 Apr 23. Nature. 356(6371):683-9. Lemmon MA. Flanagan JM. Hunt JF. Adair BD. Bormann BJ. Dempsey CE. Engelman DM. Glycophorin A dimerization is driven by specific interactions between transmembrane alpha-helices. 1992 Apr 15. J Biol Chem. 267(11):7683-9. National Institute of General Medical Sciences