Dual signaling regulated by calcyon, a D1 dopamine receptor interacting protein. Journal   Science. Citation   Science. 287(5458):1660-4 Publication date   2000 Mar 3 Authors   Lezcano N Mrzljak L Eubanks S Levenson R Goldman-Rakic P Bergson C Investigators   Robert Levenson Grant agencies   National Institute of Mental Health Grants   NIMH MH56608 NIMH P50 MH44866 MeSH headings   Membrane Proteins Prefrontal Cortex Pyramidal Cells Receptor Cross-Talk Receptors, Dopamine D1 MeSH qualifiers   metabolism Abstract   The synergistic response of cells to the stimulation of multiple receptors has been ascribed to receptor cross talk; however, the specific molecules that mediate the resultant signal amplification have not been defined. Here a 24-kilodalton single transmembrane protein, designated calcyon, we functionally characterize that interacts with the D1 dopamine receptor. Calcyon localizes to dendritic spines of D1 receptor-expressing pyramidal cells in prefrontal cortex. These studies delineate a mechanism of Gq- and Gs-coupled heterotrimeric GTP-binding protein-coupled receptor cross talk by which D1 receptors can shift effector coupling to stimulate robust intracellular calcium (Ca2+i) release as a result of interaction with calcyon. The role of calcyon in potentiating Ca2+-dependent signaling should provide insight into the D1 receptor-modulated cognitive functions of prefrontal cortex. Medline ID   20165040