Monitoring nuclear import with GFP-variant fusion proteins in digitonin-permeabilized cells. Journal   BioTechniques. Citation   Biotechniques. 31(4):772-5 Publication date   2001 Oct Authors   Petrulis JR Perdew GH Investigators   Gary Perdew Grant agencies   National Institute of Environmental Health Sciences Grants   NIEHS ES04869 MeSH headings   Active Transport, Cell Nucleus Luminescent Proteins MeSH qualifiers   metabolism Abstract   A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-variant fusion proteins allows for the rapid assessment of optimal digitonin concentration and permits nuclear import to be monitored with minimal sample preparation in real time. Medline ID   21535405