A tool-kit for cDNA microarray and promoter analysis.
Journal
  Bioinformatics (Oxford, England)
Citation
  Bioinformatics. 19(14):1846-8
Publication date
  2003 Sep 22
Authors
  Shah NH
King DC
Shah PN
Fedoroff NV
Investigators
  Nina Fedoroff
MeSH headings
  Algorithms
Arabidopsis
Expressed Sequence Tags
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
Promoter Regions (Genetics)
Sequence Alignment
Software
MeSH qualifiers
  genetics
methods
Abstract
  We describe two sets of programs for expediting routine tasks in analysis of cDNA microarray data and promoter sequences. The first set permits bad data points to be flagged with respect to a number of parameters and performs normalization in three different ways. It allows combining of result files into comprehensive data sets, evaluation of the quality of both technical and biological replicates and row and/or column standardization of data matrices. The second set supports mapping ESTs in the genome, identifying the corresponding genes and recovering their promoters, analyzing promoters for transcription factor binding sites, and visual representation of the results. The programs are designed primarily for Arabidopsis thaliana researchers, but can be adapted readily for other model systems. Availability and Supplementary information: http://www.personal.psu.edu/nhs109/Programs/
Medline ID
  22873304